LARGE1 Antibody

• Gene Name:

  LARGE xylosyl- and glucuronyltransferase 1

• Gene Aliases:

  Acetylglucosaminyltransferase-like 1A; acetylglucosaminyltransferase-like protein; Glycosyltransferase-like protein; glycosyltransferase-like protein LARGE1; LARGE; LARGE xylosyl- and glucuronyltransferase 1; MDC1D; MDDGA6; MDDGB6.

• Gene ID:

  9215

• Accession Number:

  NP_004728.1

• Reactivity:

  Human

• Immunogen:

  Range: Thr25 - Ala178

• Target:

  Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4- (phosphate-6-) mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:22223806) . Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity and plays a key role in skeletal muscle function and regeneration. LARGE elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure initiated by B3GNT1/B4GAT1 by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide (PubMed:25279699) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

• Purification:

  Affinity Purified; Protein A Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.66 mg/ml

• Format:

  Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

• Reconstitution:

  2°C to 8°C|-20°C

• Molecular Weight:

  82 kDa

• Fragment:

  IgG

• Specificity:

  The antibody is a rabbit polyclonal antibody raised against LARGE. It has been selected for its ability to recognize LARGE in immunohistochemical staining and western blotting.

• NCBI Gene Symbol:

  LARGE1

• Host or Source:

  Rabbit

• Protein Name:

  LARGE xylosyl- and glucuronyltransferase 1

• Gene Name URL:

  LARGE1

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_004737