DDB2 Antibody

CAT: 0247-OACD02997-1MLSize: 1 mLDry Ice: NoHazardous: No
CAT#:0247-OACD02997-1MLSize:1 mL
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CAS Number
9007-83-4
Gene Name
Damage specific DNA binding protein 2
Gene Aliases
Damage-specific DNA binding protein 2, 48kDa; Damage-specific DNA-binding protein 2; DDB p48 subunit; DDBB; DNA damage-binding protein 2; UV-damaged DNA-binding protein 2; UV-DDB 2; UV-DDB2; xeroderma pigmentosum group E protein; XPE.
Gene ID
1643
Accession Number
NP_000098.1
Reactivity
Human|Mouse|Rat
Immunogen
Range: Pro166 - Met383
Target
Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex) . The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1) . The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
Purification
Affinity Purified; Protein A Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Reconstitution
2°C to 8°C|-20°C
Molecular Weight
17 kDa
Fragment
IgG
Specificity
The antibody is a rabbit polyclonal antibody raised against DDB2. It has been selected for its ability to recognize DDB2 in immunohistochemical staining and western blotting.
NCBI Gene Symbol
DDB2
Host or Source
Rabbit
Protein Name
DNA damage-binding protein 2
Gene Name URL
DDB2
Nucleotide Accession Number
NM_000107

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