PTTG1 Antibody

• Gene Name:

  PTTG1 regulator of sister chromatid separation, securin

• Gene Aliases:

  EAP1; Esp1-associated protein; ESP1-associated protein 1; HPTTG; pituitary tumor-transforming 1; pituitary tumor-transforming gene 1 protein; PTTG; securin; tumor-transforming protein 1; TUTR1.

• Gene ID:

  9232

• Accession Number:

  NP_001269311.1

• Reactivity:

  Human

• Immunogen:

  Range: Ala2 - Ile202

• Target:

  Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

• Purification:

  Affinity Purified; Protein A Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Format:

  Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

• Reconstitution:

  2°C to 8°C|-20°C

• Molecular Weight:

  22 kDa

• Fragment:

  IgG

• Specificity:

  The antibody is a rabbit polyclonal antibody raised against PTTG1. It has been selected for its ability to recognize PTTG1 in immunohistochemical staining and western blotting.

• NCBI Gene Symbol:

  PTTG1

• Host or Source:

  Rabbit

• Protein Name:

  Securin

• Gene Name URL:

  PTTG1

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001282382