PHF8 Antibody

• Gene Name:

  PHD finger protein 8|PHD finger protein 8, pseudogene

• Gene Aliases:

  [histone H3]-dimethyl-L-lysine (36) demethylase PHF8; [histone H3]-dimethyl-L-lysine (9) demethylase PHF8;4921501E09Rik;9830141C09Rik; histone lysine demethylase PHF8; KIAA1111-like protein; mKIAA1111; PHD finger protein 8; PHF8.

• Gene ID:

  320595|74042

• Accession Number:

  NP_001009544.1

• Reactivity:

  Human|Mouse|Rat

• Immunogen:

  Range: Phe195 - Lys351

• Target:

  Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1) . Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

• Purification:

  Affinity Purified; Protein A Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Format:

  Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

• Reconstitution:

  2°C to 8°C|-20°C

• Molecular Weight:

  53 kDa

• Fragment:

  IgG

• Specificity:

  The antibody is a rabbit polyclonal antibody raised against PHF8. It has been selected for its ability to recognize PHF8 in immunohistochemical staining and western blotting.

• NCBI Gene Symbol:

  Phf8|Phf8-ps

• Host or Source:

  Rabbit

• Protein Name:

  Histone lysine demethylase PHF8

• Gene Name URL:

  Phf8|Phf8-ps

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_001009544