TOP1MT Antibody

• Gene Name :

  DNA topoisomerase I mitochondrial

• Gene Aliases :

  DNA topoisomerase 1, mitochondrial; DNA topoisomerase I, mitochondrial; mitochondrial topoisomerase I; topoisomerase (DNA) I, mitochondrial.

• Gene ID :

  300029

• Accession Number :

  NP_001002798.1

• Reactivity :

  Mouse|Rat

• Immunogen :

  Range: Met1 - Thr238

• Target :

  Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (3'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) .

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

• Purification :

  Affinity Purified; Protein A Purified

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  0.5 mg/ml

• Format :

  Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

• Reconstitution :

  2°C to 8°C|-20°C

• Molecular Weight :

  69 kDa

• Fragment :

  IgG

• Specificity :

  The antibody is a rabbit polyclonal antibody raised against TOP1MT. It has been selected for its ability to recognize TOP1MT in immunohistochemical staining and western blotting.

• NCBI Gene Symbol :

  Top1mt

• Host or Source :

  Rabbit

• Protein Name :

  DNA topoisomerase I, mitochondrial

• Gene Name URL :

  Top1mt

• CAS Number :

  9007-83-4

• Nucleotide Accession Number :

  NM_001002798