TOP1MT Antibody

• Gene Name:

  DNA topoisomerase 1, mitochondrial

• Gene Aliases:

  2900052H09Rik; DNA topoisomerase I, mitochondrial; mitochondrial topoisomerase I.

• Gene ID:

  72960

• Accession Number:

  NP_082680.1

• Reactivity:

  Mouse|Rat

• Immunogen:

  Range: Arg11 - Thr238

• Target:

  Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (3'-phosphotyrosyl) -enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

• Purification:

  Affinity Purified; Protein A Purified

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  0.5 mg/ml

• Format:

  Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

• Reconstitution:

  2°C to 8°C|-20°C

• Molecular Weight:

  69 kDa

• Fragment:

  IgG

• Specificity:

  The antibody is a rabbit polyclonal antibody raised against TOP1MT. It has been selected for its ability to recognize TOP1MT in immunohistochemical staining and western blotting.

• NCBI Gene Symbol:

  Top1mt

• Host or Source:

  Rabbit

• Protein Name:

  DNA topoisomerase I, mitochondrial

• Gene Name URL:

  Top1mt

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_028404