GPR183 Antibody

9007-83-4

G protein-coupled receptor 183

EBI2; EBV-induced G-protein coupled receptor 2; Epstein-Barr virus induced gene 2 (lymphocyte-specific G protein-coupled receptor) ; epstein-Barr virus-induced G-protein coupled receptor 2; G-protein coupled receptor 183; hEBI2.

1880

NP_004942

Human

Recombinant Human G-protein coupled receptor 183 protein (1-31AA)

G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes (By similarity) . Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols (PubMed:21796212, PubMed:22875855, PubMed:22930711) . Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient (By similarity) . Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses (By similarity) . Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions (By similarity) . Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity) . Collaborates with CXCR5 to mediate B-cell migration; probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5 (PubMed:22913878) . Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand (By similarity) . Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand (By similarity) . Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses: GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4 (+) dendritic cells in bridging channels (By similarity) . Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages (PubMed:25297897) . Promotes osteoclast precursor migration to bone surfaces (By similarity) . Signals constitutively through G (i) -alpha, but not G (s) -alpha or G (q) -alpha (PubMed:21673108, PubMed:25297897) . Signals constitutively also via MAPK1/3 (ERK1/2) (By similarity) .

Unconjugated

Polyclonal Antibody

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry

Protein G Purified

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Liquid

-20°C or -80°C

IgG

GPR183

Rabbit

G-protein coupled receptor 183

GPR183