NEUROD1 Antibody

• Gene Aliases:

  T2D, BETA2, BHF-1, MODY6, NEUROD, bHLHa3

• Gene ID:

  4760

• Swiss Prot:

  Q13562

• Accession Number:

  NP_002491

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human NEUROD1, corresponding to a region within N-terminal amino acids.

• Target:

  NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuronal activity results in CaMKII-mediated phosphorylation of NeuroD at Ser336, which is necessary for formation and growth of dendrites. NeuroD is also phosphorylated at Ser274 though the results are context dependent as phosphorylation by Erk stimulates NeuroD activity in pancreatic beta-cells while phosphorylation by GSK-3-beta inhibits NeuroD in neurons. NeuroD is crucially important in both the pancreas and developing nervous system, and plays a large role in the development of the inner ear and mammalian retina. Mice lacking NeuroD become severely diabetic and die shortly after birth due to defects in β-cell differentiation. The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC, IF, ICC, P-ELISA

• Purification:

  Purified by peptide affinity chromatography

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/mL

• Format:

  Liquid// PBS (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Reconstitution:

  Store at -20° C. Stable for 12 months from date of receipt.

• Molecular Weight:

  40 kDa

• Fragment:

  IgG

• Specificity:

  NEUROD1 Antibody detects endogenous levels of total NEUROD1.

• NCBI Gene Symbol:

  NEUROD1

• Host or Source:

  Rabbit

• Protein Name:

  Neurogenic differentiation factor 1

• Gene Name URL:

  NEUROD1

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_002500