MAP3K3 Antibody

• Gene Name:

  Mitogen-activated protein kinase kinase kinase 3

• Gene Aliases:

  MEKK3, MAPKKK3

• Gene ID:

  4215

• Swiss Prot:

  Q99759

• Accession Number:

  NP_002392.2NP_976226.1

• Reactivity:

  Human, Monkey

• Immunogen:

  This Aviva monoclonal antibody is produced by immunizing mice with a polypeptide (Aviva SEAL mAb technology) corresponding to surface multi-epitopes of human MEKK3.

• Target:

  This gene product is a 657-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB) -dependent reporter gene, consistent with a role in the SAPK pathway.

• Clonality:

  Monoclonal

• Type:

  Monoclonal Antibody

• Source:

  Mouse mAb

• Applications:

  WB

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Molecular Weight:

  74 kDa

• Fragment:

  Mouse IgG

• Specificity:

  MEKK3 monoclonal antibody detects endogenous levels of MEKK3 protein.

• Protein Length:

  657

• NCBI Gene Symbol:

  MAP3K3

• Host or Source:

  Mouse

• Protein Name:

  Mitogen-activated protein kinase kinase kinase 3

• Gene Name URL:

  MAP3K3

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_002401.3NM_203351.1