DOCK1 Antibody

• Gene Name:

  Dedicator of cytokinesis 1

• Gene Aliases:

  180 kDa protein downstream of CRK; ced5; dedicator of cytokinesis protein 1; DOCK180; DOwnstream of CrK.

• Gene ID:

  1793

• Swiss Prot:

  Q14185

• Accession Number:

  NP_001371.1

• Reactivity:

  Human|Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human DOCK1.

• Target:

  Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed:19004829) . Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 (PubMed:8657152) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Western blot

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid

• Reconstitution:

  -20°C

• Molecular Weight:

  215 kDa

• Notes:

  Formerly GWB-ASE520

• Specificity:

  DOCK1 antibody detects endogenous levels of total DOCK1 protein.

• NCBI Gene Symbol:

  DOCK1

• Host or Source:

  Rabbit

• Protein Name:

  Dedicator of cytokinesis protein 1

• Gene Name URL:

  DOCK1

• CAS Number:

  9007-83-4