MARK4 Antibody

• Gene Name:

  Microtubule affinity regulating kinase 4

• Gene Aliases:

  Epididymis secretory sperm binding protein; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase like 1; MAP/microtubule affinity-regulating kinase-like 1; MARK4 serine/threonine protein kinase; MARK4L; MARK4S; MARKL1; MARKL1L; PAR-1D.

• Gene ID:

  57787

• Swiss Prot:

  Q96L34

• Accession Number:

  NP_001186796.1

• Reactivity:

  Human|Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human MARK4.

• Target:

  Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942) . Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:14594945, PubMed:23666762) . Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945) . Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532) . Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999) . Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999) . Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532) . Reduces neuronal cell survival (PubMed:15009667) . Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity) . Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity) . Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid

• Reconstitution:

  -20°C

• Molecular Weight:

  82 kDa

• Notes:

  Formerly GWB-ASD832

• Specificity:

  MARK4 antibody detects endogenous levels of total MARK4 protein.

• NCBI Gene Symbol:

  MARK4

• Host or Source:

  Rabbit

• Protein Name:

  MAP/microtubule affinity-regulating kinase 4

• Gene Name URL:

  MARK4

• CAS Number:

  9007-83-4