MARK4 Antibody

9007-83-4

Microtubule affinity regulating kinase 4

Epididymis secretory sperm binding protein; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase like 1; MAP/microtubule affinity-regulating kinase-like 1; MARK4 serine/threonine protein kinase; MARK4L; MARK4S; MARKL1; MARKL1L; PAR-1D.

57787

Q96L34

NP_001186796.1

Human|Mouse

The antiserum was produced against synthesized peptide derived from human MARK4.

Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942) . Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:14594945, PubMed:23666762) . Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945) . Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532) . Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999) . Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999) . Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532) . Reduces neuronal cell survival (PubMed:15009667) . Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity) . Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity) . Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942) .

Polyclonal Antibody

Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

The optimal working dilution should be determined by the end user.

Liquid

-20°C

82 kDa

Formerly GWB-ASD832

MARK4 antibody detects endogenous levels of total MARK4 protein.

MARK4

Rabbit

MAP/microtubule affinity-regulating kinase 4