MARK1 Antibody

• Gene Name:

  Microtubule affinity regulating kinase 1|microtubule affinity regulating kinase 2|microtubule affinity regulating kinase 3|microtubule affinity regulating kinase 4

• Gene Aliases:

  Cdc25C-associated protein kinase 1; CTAK1; C-TAK1; ELKL motif kinase 1; ELKL motif kinase 2; EMK1; EMK-1; EMK-2; epididymis secretory sperm binding protein; KP78; MAP/microtubule affinity-regulating kinase 1; MAP/microtubule affinity-regulating kinase 2; MAP/microtubule affinity-regulating kinase 3; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase like 1; MAP/microtubule affinity-regulating kinase-like 1; MARK; MARK4 serine/threonine protein kinase; MARK4L; MARK4S; MARKL1; MARKL1L; PAR-1; PAR1 homolog; PAR1 homolog b; PAR1 homolog c; Par-1a; PAR1A; Par1b; Par-1b; Par1c; Par-1c; PAR-1D; protein kinase STK10; ser/Thr protein kinase PAR-1; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK; serine/threonine-protein kinase MARK1; serine/threonine-protein kinase MARK2; serine/threonine-protein kinase p78; testicular tissue protein Li 117; VIPB.

• Gene ID:

  2011|4139|4140|57787

• Swiss Prot:

  P27448|Q7KZI7|Q96L34|Q9P0L2

• Accession Number:

  NP_061120.3

• Reactivity:

  Human|Monkey|Mouse|Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human MARK1/2/3/4.

• Target:

  Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942) . Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:14594945, PubMed:23666762) . Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945) . Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532) . Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999) . Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999) . Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532) . Reduces neuronal cell survival (PubMed:15009667) . Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity) . Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity) . Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942) .|Serine/threonine-protein kinase (PubMed:23666762) . Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762) . Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3) . Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.|Serine/threonine-protein kinase (PubMed:23666762) . Involved in cell polarity and microtubule dynamics regulation. Phosphorylates DCX, MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762) . Involved in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3) .|Serine/threonine-protein kinase (PubMed:23666762) . Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762) . Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613) . Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714) .

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid

• Reconstitution:

  -20°C

• Molecular Weight:

  89 kDa

• Notes:

  Formerly GWB-ASC541

• Specificity:

  MARK1/2/3/4 (Ab-215) antibody detects endogenous levels of total MARK1/2/3/4 protein.

• NCBI Gene Symbol:

  MARK1|MARK2|MARK3|MARK4

• Host or Source:

  Rabbit

• Protein Name:

  MAP/microtubule affinity-regulating kinase 3|MAP/microtubule affinity-regulating kinase 4|Serine/threonine-protein kinase MARK1|Serine/threonine-protein kinase MARK2

• Gene Name URL:

  MARK1|MARK2|MARK3|MARK4

• CAS Number:

  9007-83-4