APAF1 Antibody

• Gene Name:

  Apoptotic peptidase activating factor 1

• Gene Aliases:

  APAF-1; apoptotic protease-activating factor 1; CED4.

• Gene ID:

  317

• Swiss Prot:

  O14727-6

• Accession Number:

  NP_001151.1

• Reactivity:

  Human|Mouse|Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human APAF-1-ALT.

• Target:

  Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid

• Reconstitution:

  -20°C

• Molecular Weight:

  40 kDa

• Notes:

  Formerly GWB-ASD008

• Specificity:

  APAF-1-ALT antibody detects endogenous levels of total APAF-1-ALT protein.

• NCBI Gene Symbol:

  APAF1

• Host or Source:

  Rabbit

• Protein Name:

  Apoptotic protease-activating factor 1

• Gene Name URL:

  APAF1

• CAS Number:

  9007-83-4