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APAF1 Antibody

CAT: 0247-OAAF01733-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OAAF01733-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Apoptotic peptidase activating factor 1
Gene Aliases
APAF-1; apoptotic protease-activating factor 1; CED4.
Gene ID
317
Swiss Prot
O14727-6
Accession Number
NP_001151.1
Reactivity
Human|Mouse|Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human APAF-1-ALT.
Target
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Dilution
The optimal working dilution should be determined by the end user.
Format
Liquid
Reconstitution
-20°C
Molecular Weight
40 kDa
Notes
Formerly GWB-ASD008
Specificity
APAF-1-ALT antibody detects endogenous levels of total APAF-1-ALT protein.
NCBI Gene Symbol
APAF1
Host or Source
Rabbit
Protein Name
Apoptotic protease-activating factor 1
Gene Name URL
APAF1

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