CCND3 Antibody

• Gene Name :

  Cyclin D3

• Gene Aliases :

  D3-type cyclin; G1/S-specific cyclin-D3.

• Gene ID :

  896

• Swiss Prot :

  P30281

• Accession Number :

  NP_001129489.1

• Reactivity :

  Human|Monkey|Mouse|Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human Cyclin D3.

• Target :

  Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

• Clonality :

  Polyclonal

• Type :

  Polyclonal Antibody

• Applications :

  Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification :

  The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration :

  1 mg/ml

• Dilution :

  The optimal working dilution should be determined by the end user.

• Format :

  Liquid.// PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Reconstitution :

  -20°C

• Molecular Weight :

  32 kDa

• Notes :

  Formerly GWB-ASC130

• Fragment :

  IgG

• Specificity :

  Cyclin D3 (Ab-283) antibody detects endogenous levels of total Cyclin D3 protein.

• NCBI Gene Symbol :

  CCND3

• Host or Source :

  Rabbit

• Protein Name :

  G1/S-specific cyclin-D3

• Gene Name URL :

  CCND3

• CAS Number :

  9007-83-4