EEF2K Antibody

• Gene Name:

  Eukaryotic elongation factor 2 kinase

• Gene Aliases:

  Alternative protein EEF2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; CaMKIII; eEF-2 kinase; eEF-2K; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase; HSU93850.

• Gene ID:

  29904

• Swiss Prot:

  O00418

• Accession Number:

  NP_037434.1

• Reactivity:

  Human|Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human eEF2K.

• Target:

  Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid// PBS (without Mg2+ and Ca2+) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol2+2+

• Reconstitution:

  -20°C

• Molecular Weight:

  82 kDa

• Notes:

  Formerly GWB-ASC091

• Fragment:

  IgG

• Specificity:

  EEF2K (Ab-366) antibody detects endogenous levels of total eEF2K protein.

• NCBI Gene Symbol:

  EEF2K

• Host or Source:

  Rabbit

• Protein Name:

  Eukaryotic elongation factor 2 kinase

• Gene Name URL:

  EEF2K

• CAS Number:

  9007-83-4