EEF2K ELISA Kit (Mouse)

CAT: 0247-OKEH02167-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH02167-96WSize:96 Tests
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CAS Number
7732-18-5
Gene Name
Eukaryotic elongation factor-2 kinase
Gene Aliases
C86191; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor 2 kinase.
Gene ID
13631
Accession Number
NP_001254639.1
Reactivity
Mouse, Mus musculus
Target
Eukaryotic elongation factor 2 kinase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology EEF2K ELISA Kit (Mouse) (OKEH02167) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EEF2K has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EEF2K is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EEF2K captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
0.09 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
82 kDa
Specificity
Natural and recombinant Mouse Eukaryotic elongation factor 2 kinase
Protein Length
724
NCBI Gene Symbol
Eef2k
Protein Name
O08796
Gene Name URL
Eef2k
Nucleotide Accession Number
NM_001267710.1

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