EIF4B Antibody (Phospho-Ser422)

• Gene Name:

  Eukaryotic translation initiation factor 4B|Janus kinase 1

• Gene Aliases:

  AIIDE; EIF-4B; eukaryotic translation initiation factor 4B; JAK1A; JAK1B; JTK3; PRO1843; tyrosine-protein kinase JAK1.

• Gene ID:

  1975|3716

• Swiss Prot:

  P23588

• Accession Number:

  NP_001408.2

• Reactivity:

  Human|Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422.

• Target:

  Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

• Clonality:

  Polyclonal

• Type:

  Polyclonal Antibody

• Applications:

  Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid

• Reconstitution:

  -20°C

• Molecular Weight:

  69 kDa

• Notes:

  Formerly GWB-ASB263

• Specificity:

  EIF4B (Phospho-Ser422) antibody detects endogenous levels of eIF4B only when phosphorylated at serine 422.

• NCBI Gene Symbol:

  EIF4B|JAK1

• Host or Source:

  Rabbit

• Protein Name:

  Eukaryotic translation initiation factor 4B

• Gene Name URL:

  EIF4B|JAK1

• CAS Number:

  9007-83-4