(S) -nitro-Blebbistatin

• Target:

  Others

• Related Pathways:

  Others

• Bioactivity:

  (S) -nitro-Blebbistatin is a more stable form of (-) -blebbistatin , which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases. (-) -Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 ?M), while poorly inhibiting smooth muscle myosin (IC50 = 80 ?M) . Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications. The addition of a nitro group stabilizes the molecule to circumvent its degradation by prolonged blue light exposure. (S) -nitro-Blebbistatin has the same stereochemistry as the active (-) -blebbistatin enantiomer.

• Smiles:

  O[C@]12C(N(CC1)C3=CC=CC=C3)=NC=4C(C2=O)=CC=C(N(=O)=O)C4

• Molecular Formula:

  C17H13N3O4

• Molecular Weight:

  323.3

• Shipping Conditions:

  Cool pack

• Storage Temperature:

  -20°C

• CAS Number:

  856925-75-2