TCEP (hydrochloride)

TCEP (hydrochloride)

• CAS Number: 51805-45-9
• UNSPSC Description: TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a non-thiol reducing agent that is more stable and produces a faster S-S reductive reaction than other chemical reductants. TCEP hydrochloride is a trialkylphosphine, selectively reduces protein disuldes without altering the properties or interacting with thiol-directed agents in the reaction mixture. TCEP hydrochloride is also a commonly used reducing agent in the DNA/AuNP chemistry[1][2][3][4].
• Target Antigen: Others
• Type: Reference compound
• Related Pathways: Others
• Field of Research: Others
• Assay Protocol: https://www.medchemexpress.com/tcep-hydrochloride.html
• Purity: 99.79
• Solubility: DMSO : 100 mg/mL (ultrasonic)|H2O : 50 mg/mL (ultrasonic;adjust pH to 7 with NaOH)
• Smiles: O=C(O)CCP(CCC(O)=O)CCC(O)=O.Cl
• Molecular Weight: 286.65
• References & Citations: [1]Dieguez-Acuña FJ, et al. Inhibition of NF-kappaB-DNA binding by mercuric ion: utility of the non-thiol reductant, tris(2-carboxyethyl)phosphine hydrochloride (TCEP), on detection of impaired NF-kappaB-DNA binding by thiol-directed agents. Toxicol In Vitro. 2000 Feb;14(1):7-16.|[2]Duchardt F, et al. A cell-penetrating peptide derived from human lactoferrin with conformation-dependent uptake efficiency. J Biol Chem. 2009 Dec 25;284(52):36099-108.|[3]Sequeira MA, et al. Modulating amyloid fibrillation in a minimalist model peptide by intermolecular disulfide chemical reduction. Phys Chem Chem Phys. 2019 Jun 5;21(22):11916-11923.|[4]Wu R, et al. Effects of Small Molecules on DNA Adsorption by Gold Nanoparticles and a Case Study of Tris(2-carboxyethyl)phosphine (TCEP). Langmuir. 2019 Oct 15;35(41):13461-13468.|[5]Han JC, Han GY. A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothreitol. Anal Biochem. 1994;220(1):5-10.
• Shipping Conditions: Room Temperature
• Storage Conditions: 4°C (Powder, protect from light, stored under nitrogen)
• Clinical Information: No Development Reported