Bcl-X Antibody

CAT: 0013-GTR18575487Size: 7 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18575487Size:7 mL

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Description

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.

UniProt

Q07817

Reactivity

Human

Immunogen

Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this anti-Bcl-X antibody.

Clonality

Monoclonal

Clone

SPM165

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the anti-Bcl-X antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG2a, kappa

UniProtKB · Q07817

Bcl-2-like protein 1

B2CL1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q07817
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: BCL2L1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: Bcl2-L-1
EC number: —
Processing: —
Secondary accessions: E1P5L6, Q5CZ89, Q5TE65, Q92976

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisEndocytosis

Cellular component

CytoplasmCytoplasmic vesicleCytoskeletonMembraneMitochondrionMitochondrion inner membraneMitochondrion outer membraneNucleusSynapse

PTM

PhosphoproteinUbl conjugation

Domain

TransmembraneTransmembrane helix

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Alternative Products

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GWB-3DEA90	Bcl-X, Antibody
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