Caspase-3 Substrate DEVD-AFC

CAT:

26-1007-200

Size:

200 Assays

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Description: Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Shipping Conditions: gel pack
Storage Conditions: -20°C
Shelf Life: 6-12 months

Cat	Name
MBS566149-01	Ac-DEVD-AFC [Caspase-3 Substrate (AFC)]
MBS566149-02	Ac-DEVD-AFC [Caspase-3 Substrate (AFC)]
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C5524-5	Ac-DEVD-AFC
T36332-1mg	Z-DEVD-AFC
HY-P1986-01	Z-DEVD-AFC
HY-P1005-03	Ac-DEVD-AFC
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HY-P1005-01	Ac-DEVD-AFC