Ac-DEVD-AFC [Caspase-3 Substrate (AFC)]

• Storage Conditions:

  For long term storage, store at -20 °C. Protect from light

• Specificity:

  Fluorogenic substrate for caspase-3 (CPP32), with a Km=9.7uM) and related cysteine proteases. Sequence is based on PARP cleavage at Asp216 for caspase-3. Similar to Ac-DEVD-AMC but the AFC fluorophore has a greater Stokes' shift upon cleavage than AMC. Reaction can be monitored quantitatively or visually using a hand-held long-UV lamp and visualizing a blue to green shift in fluorescence upon cleavage. Ex.: 400nm, Em.: 505nm

• Short Name:

  [Ac-DEVD-AFC]