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Thrombin Sepharose Beads

CAT: 0026-7925-25Size: 25 mLDry Ice: NoHazardous: No
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CAT#:0026-7925-25Size:25 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Thrombin Sepharose beads are designed for efficient cleavage of recombinant fusion proteins, containing a thrombin cleavage site (e.g. Leu-Val-Pro-Arg-ll-Gly-Ser; ll: cleavage site), circumventing the need for chromatographic techniques to remove thrombin after cleavage reaction is completed. BioVision’s Thrombin Sepharose is prepared by covalent coupling of bovine thrombin to activated 6% cross-linked Sepharose beads. 15 µl of the slurry is sufficient to cleave >90% of 1 mg of BioVision’s 6xHis-tagged fusion protein in 50 mM Tris buffer, 0.1 M NaCl, pH 8.0 within 4 h at room temperature. These beads can be regenerated for repeat use. The reusability depends on multiple criteria including the usage and handling of the product.
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

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