Acid Sphingomyelinase Assay Kit II (Colorimetric)
CAT:
26-K192-100
Size:
100 Assays
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: Yes
Acid Sphingomyelinase Assay Kit II (Colorimetric)
- Description: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyelinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples.
- Reactivity: All
- Applications: Measurement of ASMase activity in various tissues/cell extracts
- Detection Method: Colorimetric (OD= 570 nm)
- Features & Benefits: Complete set of reagents for the detection of Acid SMase in biological samples,Simple, specific (detect acid SMase activity only)
- Shipping Conditions: Dry Ice
- Storage Conditions: -80°C
- Shelf Life: 12 months