Sphingomyelinase Activity Fluorometric Assay Kit

Sphingomyelinase Activity Fluorometric Assay Kit

• Description: Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation and sterol homeostasis. BioVision’s SMase Fluorometric Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using fluorescence (Ex/Em = 535/587 nm). Neutral sphingomyelinase (N-SMase) is a mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce fluorescence. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 10 µU/ml in a variety of samples.
• Applications: Measurement of SMase activity in various tissues/cells extracts
• Detection Method: Fluorescence (Ex/Em 535/587 nm)
• Features & Benefits: • Simple, rapid & convenient • The assay can detect sphingomyleinase activity as low as 10 µU/ml in a variety of samples.
• Shipping Conditions: gel pack
• Storage Conditions: -20°C
• Shelf Life: 12 months