NOS2 / iNOS (mouse) ELISA Kit

NOS2 / iNOS (mouse) ELISA Kit

• Description: Nitric oxide synthases (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s NOS2 ELISA kit is a sandwich ELISA assay for the quantitative measurement of NOS2 in mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of NOS2 captured from the samples.
• CAS Number: 7732-18-5
• Reactivity: Mouse
• Applications: For quantitative detection of NOS2/iNOS in serum, plasma, tissue homogenates and other biological fluids.,Sensitivity: < 0.188 ng/ml,Detection Range: 0.312 - 20 ng/ml
• Detection Method: colormetric
• Features & Benefits: Sensitivity: < 0.188 ng/ml,Detection method- Absorbance (450 nm),Detection Range: 0.312-20 ng/ml,Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%,Application- quantitative measurement of NOS2 in serum, plasma, tissue homogenates and other biological fluids.,No significant cross-reactivity or interference was observed,Species reactivity- mouse
• Shipping Conditions: Gel Pack
• Storage Conditions: 4ºC
• Shelf Life: 6 months