Renin (Mouse) ELISA Kit

Renin (Mouse) ELISA Kit

• Description: Renin is a highly specific endopeptidase with only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. BioVision's Renin ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to mouse Renin. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for mouse Renin and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of the mouse Renin. The concentration of Mouse Renin in the samples can be calculated by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Reactivity: Mouse
• Detection Method: Absorbance (450 nm)
• Features & Benefits: Sensitivity: 0.10 ng/ml, Detection Range: 0.16—10 ng/ml, Specificity: This kit recognizes Mouse Renin in samples. No significant cross-reactivity or interference between Mouse Renin and analogues was observed.
• Shipping Conditions: Gel Pack
• Storage Conditions: 4ºC
• Shelf Life: 6 months