Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit
CAT:
26-K679-100
Size:
100 Assays
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit
- Description: Pyruvate Dehydrogenase (PDH) (EC 1.2.4.1) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress. BioVision’s PDH assay kit provides a quick and easy way for monitoring PDH activity in various samples. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.
- Applications: Measurement of pyruvate dehydrogenase activity in various tissues/cells. Analysis of cell signaling pathway
- Detection Method: Absorbance (450 nm)
- Features & Benefits: • Simple, rapid & convenient • can measure pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples
- Shipping Conditions: gel pack
- Storage Conditions: -20°C
- Shelf Life: 12 months