Pyruvate Dehydrogenase (PDH) Activity Assay Kit

Pyruvate Dehydrogenase (PDH) Activity Assay Kit

• Synonyms: PDH
• Field of Research: Tricarboxylic Acid（TCA）Cycle
• Detection Method: Colorimetric method
• Assay Principle: Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.
• Assay Performance Time: 15 min
• Detection Range: 0.28-168.91 U/L
• Sensitivity: 0.28 U /L
• Shipping Conditions: Ice bag
• Storage Conditions: This product can be stored at -20°C for 12 months with shading light.