Pyruvate Dehydrogenase (PDH) Activity Assay Kit

• Target:

  PDH

• Type:

  Colorimetric method

• Field of Research:

  Tricarboxylic Acid (TCA) Cycle

• Assay Principle:

  Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.

• Assay Performance Time:

  15 min

• Sample Type:

  Serum (plasma) ; urine; animal tissue; cell

• Detection Range:

  0.28-168.91 U/L

• Sensitivity:

  0.28 U /L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  10%

• Intra CV:

  2%

• Detection Instrument:

  Microplate reader