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PD-1 (human) ELISA Kit

CAT: 0026-K4153-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K4153-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human PD-1 (Programmed Death-1) ELISA kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of human PD-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human PD-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
CAS Number
7732-18-5
Reactivity
Human
Applications
This ELISA kit is used for quantitative measurement of PD-1
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to measure the level of PD-1 in serum, plasma, tissue homogenates, culture supernatants and other biological fluids. Detection Range: 0.156 - 10 ng/ml Sensitivity: < 0.094 ng/ml
Shipping Conditions
gel pack
Storage Conditions
4°C
Shelf Life
6 months