Cell Surface Protein Isolation Kit

CAT: 0026-K295-10Size: 10 ReactionsDry Ice: NoHazardous: No
CAT#:0026-K295-10Size:10 Reactions
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Description
Cell surface proteins play a major role in signal transduction, cell adhesion, transport and serve as diagnostic and pharmacological targets. BioVision’s Cell Surface Protein Isolation Kit provides a simple and efficient method for the isolation of cell surface proteins. In this method, cells are first labeled with Sulfo-NHS-SS-Biotin, an amine-reactive, thiol-cleavable, biotinylation reagent. Cells are subsequently lysed and the labeled cell surface proteins are isolated using Streptavidin beads. The bound proteins are then released from beads by incubating with DTT solution. Biotinylation reagent is cell-membrane-impermeable with an extended spacer arm to reduce steric hindrances associated with streptavidin binding. This convenient kit provides all the required components for optimal labeling and isolation of the cell surface proteins.
Applications
Isolated cell surface proteins can be used for various downstream applications such as Western Blotting or other structural and functional studies
Detection Method
Western Blotting
Features & Benefits
• Simple and efficient method for the isolation of cell surface proteins • Includes all required components for optimal labeling and isolation of the cell surface proteins
Shipping Conditions
gel pack
Storage Conditions
4°C
Shelf Life
12 months

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