α-L-Fucosidase (FUCA1) Assay Kit (Colorimetric)

α-L-Fucosidase (FUCA1) Assay Kit (Colorimetric)

• Description: α-L-Fucosidase (EC 3.2.1.51) (FUCA1) is a hydrolase that is able to cleave α-L-fucosyl moieties from glycoconjugates and oligosaccharides. Fucosidase plays pivotal roles in cell differentiation, apoptosis, inflammation and host-pathogen interaction. Furthermore, abnormal concentrations of this glycosidase have been observed in patients suffering from cancer, fucosidosis, rheumatoid arthritis, cystic fibrosis, and leukocyte adhesion deficiency. Industrial applications of α-L-Fucosidase (FUCA1) include synthesis of fucosylated analogs that could serve as antiadhesion compounds, cancer vaccines, and anti-inflammatory therapeutics. BioVision’s α-L-Fucosidase Assay Kit provides a simple, sensitive and high-throughput adaptable approach to detect physiological concentrations of this glycosidase in a variety of biological samples. In this assay, α-L-Fucosidase uses a synthetic chloro p-nitrophenol derivative (R-pNP) as an α-L-Fucosidase substrate and releases a chlorinated pNP derivative which can be measured kinetically under acidic conditions (OD 405 nm). The assay involves a one-step simple reaction, with minimal sample preparation and does not need a stop solution to complete the reaction. The assay can detect as low as 20 µU of α-L-Fucosidase activity in a variety of samples.
• Reactivity: Mammalian
• Applications: Estimation of FUCA1/AFU in various biological samples.
• Detection Method: Detection method- Absorbance (OD = 405 nm).
• Features & Benefits: • Simple, fast and convenient assay • Can detect as low as 20 µU of α-L-Fucosidase activity in a variety of samples.
• Shipping Conditions: gel pack
• Storage Conditions: -20°C
• Shelf Life: 12 months