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EMI-1 Antibody

CAT: 0013-GTR18574026Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18574026Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
UniProt
Q9UKT4
Reactivity
Human
Immunogen
A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.
Clonality
Monoclonal
Clone
FBXP5
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the EMI-1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2a, kappa

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