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Recombinant CD86 Antibody

CAT: 0013-GTR18573451Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18573451Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
UniProt
P42081
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the recombinant CD86 antibody.
Clonality
Recombinant
Clone
C86/2160R
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein A affinity chromatography
Dilution
ELISA: 2-4ug/ml (order BSA/azide-free format), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant CD86 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
ELISA, FACS, IF, IHC-P, WB
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa

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