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Myogenin Antibody

CAT: 0013-GTR18573121Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18573121Size:100 µg
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Description
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6) . MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
UniProt
P15173
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.
Clonality
Monoclonal
Clone
F5D
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
ELISA (order BSA/sodium azide-free format for coating), Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the Myogenin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
ELISA, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

UniProtKB · P15173

Myogenin

MYOG_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15173
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MYOG
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q53XW6
Protein keywords

Molecular function

ActivatorDevelopmental proteinDNA-binding

Biological process

Cell cycleDifferentiationMyogenesisTranscriptionTranscription regulation

Cellular component

Nucleus

PTM

Phosphoprotein

Technical term

Proteomics identificationReference proteome

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