CD34 Antibody Cocktail

CAT: 0013-GTR18572903Size: 7 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18572903Size:7 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens) . Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but shows low specificity.

UniProt

P28906

Reactivity

Human

Immunogen

A detergent solubilized vesicular suspension prepared from human term placenta (QBEnd/10) and recombinant full-length human protein (HPCA1/763) were used as the immunogen for the CD34 antibody cocktail.

Clonality

Monoclonal

Clone

QBEnd/10 + HPCA1/763

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the CD34 antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG1, lambda

UniProtKB · P28906

Hematopoietic progenitor cell antigen CD34

CD34_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P28906
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CD34
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: A8K664, Q15970, Q15971, Q5JTA3, Q5JTA4, Q9UJB1

Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

GlycoproteinPhosphoprotein

Cellular component

Membrane

Domain

SignalTransmembraneTransmembrane helix

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