Products for Research Use Only
RegisterLogin

SM22 Antibody

CAT: 0013-GTR18572770Size: 7 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18572770Size:7 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
This mAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.
UniProt
Q01995
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the SM22 antibody.
Clonality
Monoclonal
Clone
TAGLN/247
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein G affinity chromatography
Dilution
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the SM22 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR18574118SM22 alpha Antibody
GTR18574117SM22 alpha Antibody
GTR18082340-01SM22 alpha Antibody
GTR18163769SM22 alpha Antibody
GTR18292806Transgelin (SM22-alpha) Antibody
GTR18292174Transgelin (SM22-alpha) Antibody
GTR17696531SM22 Alpha/Transgelin Antibody
GTR18291897Transgelin (SM22-alpha) Antibody
GTR18881868-01Human SM22 protein
GTR18108083-01SM22 alpha Rabbit Polyclonal Antibody

Alternative Products

CatalogName
V2877IHC-7MLSM22 Antibody
MBS9602283-01SM22 aphla Antibody
abx430896SM22 Alpha Antibody
abx238935Transgelin / SM22 Antibody
PAab08934Anti-transgelin/SM22 antibody
bsm-61818R-TRSM22 Alpha Recombinant Antibody
33-977SM22 alpha Antibody [SMP22a]
C20955-50uLTransgelin/SM22 Antibody [H9M7]
CQA2264-01Anti-SM22 alpha Antibody
abx238936Transgelin / SM22-specific Antibody

Popular Products