PSA Antibody Cocktail

CAT: 0013-GTR18572528Size: 7 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18572528Size:7 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA) . This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT) . A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

UniProt

P07288

Reactivity

Human

Immunogen

Recombinant full-length human prostate serum antigen protein was used as the immunogen for the PSA antibody cocktail.

Clonality

Monoclonal

Clone

KLK3/801 + KLK3/1248

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the PSA antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG1, kappa

UniProtKB · P07288

Prostate-specific antigen

KLK3_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P07288
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: KLK3
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: PSA
EC number: 3.4.21.77
Processing: Precursor
Secondary accessions: C9JXH3, G3V0H4, G3XAE3, Q15096, Q16272, Q86TG8, Q8IXI4

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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