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Recombinant Human Apolipoprotein B-100 (APOB), partial

CAT: 0399-CSB-EP001918HUb0-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP001918HUb0-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Apo B-100 (Apo B-48)
Abbreviation
Recombinant Human APOB protein, partial
Gene Name
APOB
UniProt
P04114
Expression Region
28-127aa
Organism
Homo sapiens (Human)
Target Sequence
EEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMS
Tag
N-terminal 10xHis-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Epigenetics and Nuclear Signaling
Relevance
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100) . Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100) . Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Molecular Weight
14.7 kDa
References & Citations
"Molecular cloning of human LDL apolipoprotein B cDNA. Evidence for more than one gene per haploid genome." Shoulders C.C., Myant N.B., Sidoli A., Rodriguez J.C., Cortese C., Baralle F.E., Cortese R. Atherosclerosis 58:277-289 (1985)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial

UniProtKB · P04114

Apolipoprotein B-100

APOB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04114
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Apo B-100
EC number
—
Processing
Precursor
Secondary accessions
O00502, P78479, P78480, P78481, Q13779, Q13785, Q13786, Q13787, Q13788, Q4ZG63, Q53QC8, Q7Z600, Q9UMN0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationDisulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Disease

AtherosclerosisDisease variant

Biological process

Cholesterol metabolismLipid metabolismLipid transportSteroid metabolismSterol metabolismTransport

Cellular component

ChylomicronCytoplasmLDLLipid dropletSecretedVLDL

Molecular function

Heparin-binding

Coding sequence diversity

RNA editing

Domain

Signal

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