Recombinant Human Sorting nexin-3 (SNX3)

• Product Name Alternative:

  Protein SDP3

• Abbreviation:

  Recombinant Human SNX3 protein

• Gene Name:

  SNX3

• UniProt:

  O60493

• Expression Region:

  3-162aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  ETVADTRRLITKPQNLNDAYGPPSNFLEIDVSNPQTVGVGRGRFTTYEIRVKTNLPIFKLKESTVRRRYSDFEWLRSELERESKVVVPPLPGKAFLRQLPFRGDDGIFDDNFIEERKQGLEQFINKVAGHPLAQNERCLHMFLQDEIIDKSYTPSKIRHA

• Tag:

  N-terminal GST-tagged

• Type:

  Developed Protein

• Source:

  E.coli

• Field of Research:

  Transport

• Relevance:

  Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns (P3) ) . Also can bind phosphatidylinositol 4-phosphate (PtdIns (P4) ), phosphatidylinositol 5-phosphate (PtdIns (P5) ) and phosphatidylinositol 3,5-biphosphate (PtdIns (3,5) P2) . Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome mbrane association of the retromer cargo-selective subcomplex (CSC/VPS) . May in part act as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway . Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G . Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes . Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor TFRC presumably by delivering the tranferrin:transferrin receptor complex to recycling endosomes; the function may involve the CSC retromer subcomplex . In the case of Salmonella enterica infection plays arole in maturation of the Salmonella-containing vacuole (SCV) and promotes recruitment of LAMP1 to SCVs .

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns (P3) ) . Also can bind phosphatidylinositol 4-phosphate (PtdIns (P4) ), phosphatidylinositol 5-phosphate (PtdIns (P5) ) and phosphatidylinositol 3,5-biphosphate (PtdIns (3,5) P2) (By similarity) . Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC/VPS) . May in part act as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway

• Molecular Weight:

  45.6 kDa

• References & Citations:

  Identification of a family of sorting nexin molecules and characterization of their association with receptors.Haft C.R., de la Luz Sierra M., Barr V.A., Haft D.H., Taylor S.I.Mol. Cell. Biol. 18:7278-7287 (1998)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein