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Fibronectin, mouse Antibody

CAT: 0013-GTR17702208Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR17702208Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Rabbit polyclonal antibody to Fibronectin
UniProt
P11276
Reactivity
Mouse
Immunogen
Purified fibronectin from murine plasma
Clonality
Polyclonal
Conjugation
Unconjugated
Source
Serum from rabbits immunized with purified mouse fibronectin.
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein G affinity purification
Concentration
App. 5 mg/ml
Dilution
IHC (frozen) : 1:1000, ELISA:1:20000 (OD 0.5) Optimal working dilutions should, however, be determined by antibody titration.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Mouse skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC 60 min at RT or 2-8°C over night
Tested Applications
ELISA, IF, IHC, IHC-P, RIA
Host or Source
Rabbit

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