Recombinant Bacillus anthracis Thymidylate kinase (tmk)

• Product Name Alternative:

  Tmk; BAMEG_0039Thymidylate kinase; EC 2.7.4.9; dTMP kinase

• Abbreviation:

  Recombinant Bacillus anthracis Thymidylate kinase protein

• Gene Name:

  Tmk

• UniProt:

  C3LJ02

• Expression Region:

  1-208aa

• Organism:

  Bacillus anthracis (strain CDC 684 / NRRL 3495)

• Target Sequence:

  MKGLFVTIEGPEGSGKTTLIQSLLPYFEQKEQKVMATREPGGIAISEDIRTILHKQEYTMMEARTEALLYAAARRQHLVEKVMPALNEDYLVLCDRFIDSSLAYQGYARGLGMDKVFEINRFATEDCMPSLTIYLDIEPEVGLARIAKDAGREVNRLDMEDISFHKRVREGYLQVVERFSDRIVLVNADQPMEKLIEEVIQVIEDKLL

• Tag:

  N-terminal 6xHis-tagged

• Type:

  In Stock Protein

• Source:

  E.coli

• Field of Research:

  Others

• Relevance:

  Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.

• Endotoxin:

  Not test

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Not Test

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Function:

  Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.

• Molecular Weight:

  27.8 kDa

• References & Citations:

  "Genome sequence of Bacillus anthracis str. CDC 684."Dodson R.J., Munk A.C., Brettin T., Bruce D., Detter C., Tapia R., Han C., Sutton G., Sims D.Submitted (OCT-2008)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length