ViraSafe™ Universal Lentiviral Expression System, Ecotropic

• Catalog number
  VPK-211-ECO
• Price
  Please ask
• Size
  1 test kit

• We send this reagant on
  Blue Ice
• The temperatureerature for storage is
  -20ºC
• Technical file
• SDS or safty data sheet
  more (VPK-205 through VPK-222-xxx) GHS.pdf
• Type of reagent
  Viral Expression
• Related techniques
  Viral Expression, Lentiviral Expression, Lentiviral Expression and Packaging, Lentiviral Expression Systems
• Use of the reagent
  Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe™ Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.
• Cellular use
  Significantly reduced homology compared to other 3rd generation expression systems for added safety, without loss of titer
• Protocol information
  Complete Expression System contains packaging plasmids and expression vector
• Reagent properties
  Cloning capacity of expression vector = 9.4 kb
• Additional lab advice
  Resulting ecotropic virus can readily infect mouse or rat cells only
• Living modified organisme in this reagent or LMO
  Not applicable
• Species compatible to
  Humans
• Use of this test kit or product
  Genetics lab
• Antigen
  a specific protein sequence
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,47
• Molecular weight
  in kilo Danlto or kDa
• This reagent is purified
  0.97

• Gene target
  ViraSafe™   Universal   Lentiviral   Expression   Ecotropic  
• Short name
  ViraSafe™ Universal Lentiviral Expression System, Ecotropic
• Alternative name
  ViraSafe™ Universal Lentiviral Expression System, Ecotropic

MeSH Data

• Name
  Virus Cultivation
• Concept
  Scope note: Process of growing viruses in live animals, plants, or cultured cells.
• Tree numbers
  • E01.370.225.875.970
  • E05.200.875.970
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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