Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit

• Catalog number
  K670-100
• Price
  Please ask
• Size
  100 assays

• Description
  Only Kit on the market to measure Triose Phosphate Isomerase (TPI) Activity
• Summary
  • Detection method- Absorbance (OD 450 nm) • Applications: - Measurement of TPI activity in various tissues and cells - Analysis of glycolysis & pentose phosphate pathway and lipid metabolism
• Detection Method
  Absorbance (OD 450 nm)
• Applications
  • Measurement of TPI activity in various tissues and cells • Analysis of glycolysis & pentose phosphate pathway and lipid metabolism
• Sample Type
  • Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: Adherent or suspension Cells • Human serum or plasma
• Features Benefits
  • Simple, highly sensitive, and high-throughput. • The assay can measure Triose Phosphate Isomerase activity as low as 40 mU/ml.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms. In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment. BioVision’s Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples. In this kit, Triose Phosphate Isomerase converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the Enzyme Mix & Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, & high-throughput and can detect Triose Phosphate Isomerase activity as low as 40 mU/ml.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Triose   Phosphate   Isomerase   TPI   Activity   Kit  
• Short name
  Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Triose Phosphate Isomerase (TPI) Activity colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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