Sirtuin 2, human recombinant

• Catalog number
  7632-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Regulatory protein SIR2 homolog 2, SIR2-like protein 2
• Alternative_names
  NAD-dependent protein deacetylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2
• Description
  A NAD-dependent deacetylase
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  ≥ 80 units/mg
• Biological activity
  Activity of SIRT2 was tested using the substrate Arg-His-Lys-Lys(Ac)-AFC, which is a fluorogenic, acetylated peptide based on residues 379-382 of p53.
• Unit Definition
  One unit of the recombinant SIRT2 is defined as the amount of enzyme that deacetylates 1 pmol of substrate Arg-His-Lys-Lys(Ac)-AFC per minute at 37°C in a reaction solution containing 50 mM Tris, pH 7.5, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mM NAD+ and 1 mg/ml BSA.
• Molecular Weight
  43 kDa
• Storage Temp
  -70°C
• Shipping
  dry ice
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  rh-Sirtuin 2 (SIRT2) with the native N-terminus is supplied as a liquid of 2 mg/ml in 25 mM Tris, pH 7.5, 100 mM NaCl, 2.7 mM KCl, 3 mM MgCl2, 10 % glycerol.
• Background Information
  Human sirtuins function as intracellular regulatory proteins. Sirtuin 2 is a member of the class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. The enzymatic activity of class III HDACs is nicotinamide adenine dinucleotide (0.1mM NAD+) dependent and insensitive to HDAC inhibitor trichostatin A.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Sirtuin  
• Gene symbol
  SIRT2, SIRT6, SIRT4
• Short name
  Sirtuin 2, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Sirtuin 2, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:10886
• Gene
  SIRT2
• Long gene name
  sirtuin 2
• Synonyms gene
  • SIR2L
• Synonyms gene name
  • sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2
  • sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• GenBank acession
  • AF083107
• Locus
  19q13.2
• Discovery year
  1998-10-14
• Entrez gene record
  22933
• Pubmed identfication
  • 10393250
  • 10381378
• Classification
  • Sirtuins
• VEGA ID
  OTTHUMG00000150480

Gene info

• Identity
  HGNC:14934
• Gene
  SIRT6
• Long gene name
  sirtuin 6
• Synonyms gene name
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
  • sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• GenBank acession
  • AF233396
• Locus
  19p13.3
• Discovery year
  2001-03-20
• Entrez gene record
  51548
• Pubmed identfication
  • 10873683
• Classification
  • Sirtuins
• VEGA ID
  OTTHUMG00000181847

Gene info

• Identity
  HGNC:14932
• Gene
  SIRT4
• Long gene name
  sirtuin 4
• Synonyms gene name
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 4
  • sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• Synonyms
  • SIR2L4
• GenBank acession
  • AF083109
• Locus
  12q24.31
• Discovery year
  2001-03-20
• Entrez gene record
  23409
• Pubmed identfication
  • 10381378
• RefSeq identity
  • NM_012240
• Classification
  • Sirtuins
• VEGA ID
  OTTHUMG00000169028

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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