Sushi Peptide S3, bacterial recombinant

• Catalog number
  7611-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Factor C Sushi 3, Sushi 3
• Alternative_names
  Factor C Sushi 3, Sushi 3
• Description
  Binds to endotoxins in mammalian cell culture systems.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Molecular Weight
  16.1 kDa
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Lyophilized from 50 mM Tris. HCl and 100 mM NaCl (pH 8.0)
• Reconstitution Instructions
  Reconstitute with water. Recommended conc: 0.1 - 1 mg/ml.
• Background Information
  Biovision’s Sushi Peptide S3 is a trimer of one of the high endotoxin-binding domains, Sushi 3 (or S3) within Factor C, a lipopolysaccharide (LPS)-sensitive serine protease of the horseshoe crab (Limulus Polyphemus). S3 display detergent-like properties in disrupting LPS aggregates, with specificity for palmitoyl-oleoyl-phosphatidylglycerol (POPG) resulting from electrostatic and hydrophobic forces between the peptides and the bacterial lipids. During interaction with POPG, the S3 resumes a mixture of alpha-helix and beta-sheet structures. The unsaturated nature of POPG confers fluidity and enhances insertion of the peptides into the lipid bilayer, causing maximal disruption of the bacterial membrane. In short, peptide S3 can bind to lipopolysaccharide (LPS) and inhibit the growth of Gram-negative bacteria without affecting mammalian cells. It has been shown that endotoxin activates Factor C-based catalytic coagulation cascade resulting in the gelation of Limulus blood. This process is the basis of Limulus Amebocyte Lysate (LAL) endotoxin detection method.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional description
  Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Sushi   Peptide   bacterial  
• Short name
  Sushi Peptide S3, bacterial recombinant
• Technique
  Recombinant, peptide, peptides, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Sushi short protein sequence S3, bacterial Rec.
• Alternative technique
  rec, peptides

MeSH Data

• Name
  Radioimmunosorbent Test
• Concept
  Scope note: Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
• Tree numbers
  • E05.478.566.380.830
  • E05.478.566.639.830
  • E05.601.470.380.830
  • E05.601.470.639.830
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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