Recombinant purified FLAG tag-Protein (~9 kda) control WB +ve control
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Catalog number
FLAG12-C
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Price
Please ask
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Size
100 ul
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Stock availability
Available
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Category
Western Blot Control
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Antibody type
N/A
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Antibody host
N/A
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Antibody conjugate
His-Tag?
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Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
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Notes
The Recombinant purified FLAG tag-Protein (~9 kda) control WB +ve control is manufactured for Research Use Only or for diagnostics purposes.
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. The kilo Daltons subunit weight of Recombinant purified tag-Protein (~9 ) control +ve control compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Recombinant purified tag-Protein (~9 ) control +ve control
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Technique
Recombinant, purified, Control, WB, controls, purifications, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. adi advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags, Western Blot on a membrane
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Label
Flag
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Species
N/A
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Alternative name
Rec. enriched FLAG detection labelled-Protein (~9 kiloDalton) reference Western Blot +ve reference
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Alternative technique
rec, pure, controls
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Tissue
control
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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