Recombinant Human DCAMKL1 / DCLK1 Protein

• Catalog number
  PKSH031076-20µg
• Price
  Please ask
• Size
  20µg

• Synonym
  CL1;CLICK1;DCAMKL1;DCDC3A;DCLK
• Activity
  NA
• Sequence
  Met 1-Val 705
• Fusion tag
  NA
• Accession
  O15075-1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
• Purity
  ＞80 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  78.5 kDa
• AP Mol Mass
  64 kDa
• Formulation
  Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
• Background
  DCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.
• Technical data
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  DCAMKL1   DCLK1   Protein  
• Gene symbol
  DCLK1
• Short name
  Recombinant DCAMKL1 / DCLK1 Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens DCAMKL1 / DCLK1 Protein
• Alternative technique
  rec

Gene info

• Identity
  HGNC:2700
• Gene
  DCLK1
• Long gene name
  doublecortin like kinase 1
• Synonyms gene
  • DCAMKL1
• Synonyms gene name
  • doublecortin and CaM kinase-like 1
  • doublecortin-like kinase 1
• Synonyms
  • KIAA0369
  • DCLK
  • DCDC3A
• GenBank acession
  • AB002367
• Locus
  13q13.3
• Discovery year
  1999-02-22
• Entrez gene record
  9201
• Pubmed identfication
  • 9747029
  • 10036192
• RefSeq identity
  • NM_004734
• Classification
  • Doublecortin superfamily
• VEGA ID
  OTTHUMG00000016729

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products