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Immunogen
A synthesized peptide derived from human Survivin
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Antibody s target
Human Survivin
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Antibody s full description
Rabbit Anti-Human Survivin Polyclonal
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Primary research fields
Neuroscience, Neurodegeneration, Cancer, Apoptosis
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
API4 Antibody, Apoptosis inhibitor 4 Antibody, Apoptosis inhibitor survivin Antibody, Baculoviral IAP repeat containing 5 Antibody, BIRC 5 Antibody, EPR 1 Antibody, IAP4 Antibody, Survivin variant 3 alpha Antibody, SVV Antibody, TIAP Antibody
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Verified applications
WB, IHC, ICC/IF
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Raised in
Rabbit
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Antibody s reactivity
Human, Mouse, Rat
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Antibody s recommended dilutions for use
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects ~16kDa, endogenous levels of total Survivin
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:1000 dilution of SPC-1335 was sufficient for detection of Survivin in 10 µg of Lung cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm, Nucleus, Chromosome
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Tissue specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
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Scientific context
Survivin, or baculovirus inhibitor of apoptosis repeat-containing 5 is a member of the inhibitor of apoptosis family (IAP). It functions to inhibit caspase activation. It may also play a role in regulating mitosis. It is regulated by the p53 protein, a direct target gene of the Wnt pathway, and is upregulated by beta-catenin.
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Bibliography
1. Altieri D.C. (1994). J. Biol. Chem. 269(5): 3139–3142. 2. Sah N.K., Khan Z., Khan G.J., Bisen P.S. (2006). Cancer Lett. 244(2): 164–171. 3. Olie R.A., et al. (2000). Cancer Res. 60(11): 2805–2809.
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Released date
6/Sep/2016
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NCBI number
NP_001159.2
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Gene number
332
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Protein number
O15392
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure legend
Western blot analysis of Mouse Lung cell lysates showing detection of ~20kDa Survivin protein using Rabbit Anti-Survivin Polyclonal Antibody (SPC-1335). Lane 1: Mouse Lung tissue lysate treated with the immunizing peptide. Lane 2: Mouse Lung tissue lysate. Primary Antibody: Rabbit Anti-Survivin Polyclonal Antibody (SPC-1335) at 1:1000. Predicted/Observed Size: ~20kDa. Rabbit Anti-Survivin Antibody used in Western blot (WB) on Lung cell lysates (SPC-1335)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
