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Rabbit Anti-Human Survivin Polyclonal: ATTO 390

  • Catalog number
    SPC-1335D-A390
  • Product name
    Rabbit Anti-Human Survivin Polyclonal: ATTO 390
  • Size
    100 µl
  • Label
    ATTO 390
  • Immunogen
    A synthesized peptide derived from human Survivin
  • Antibody s target
    Human Survivin
  • Antibody s full description
    Rabbit Anti-Human Survivin Polyclonal
  • Primary research fields
    Neuroscience, Neurodegeneration, Cancer, Apoptosis
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    API4 Antibody, Apoptosis inhibitor 4 Antibody, Apoptosis inhibitor survivin Antibody, Baculoviral IAP repeat containing 5 Antibody, BIRC 5 Antibody, EPR 1 Antibody, IAP4 Antibody, Survivin variant 3 alpha Antibody, SVV Antibody, TIAP Antibody
  • Verified applications
    WB, IHC, ICC/IF
  • Raised in
    Rabbit
  • Isotype
    IgG
  • Antibody s reactivity
    Human, Mouse, Rat
  • Antibody s recommended dilutions for use
    WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
  • Antibody s concentration
    See included datasheet or contact our support service
  • Antibody s specificity
    Detects ~16kDa, endogenous levels of total Survivin
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-1335 was sufficient for detection of Survivin in 10 µg of Lung cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Cytoplasm, Nucleus, Chromosome
  • Tissue specificity
    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Scientific context
    Survivin, or baculovirus inhibitor of apoptosis repeat-containing 5 is a member of the inhibitor of apoptosis family (IAP). It functions to inhibit caspase activation. It may also play a role in regulating mitosis. It is regulated by the p53 protein, a direct target gene of the Wnt pathway, and is upregulated by beta-catenin.
  • Bibliography
    1. Altieri D.C. (1994). J. Biol. Chem. 269(5): 3139–3142. 2. Sah N.K., Khan Z., Khan G.J., Bisen P.S. (2006). Cancer Lett. 244(2): 164–171. 3. Olie R.A., et al. (2000). Cancer Res. 60(11): 2805–2809.
  • Released date
    6/Sep/2016
  • NCBI number
    NP_001159.2
  • Gene number
    332
  • Protein number
    O15392
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure link
    Representative_figure_link
  • Representative figure legend
    Western blot analysis of Mouse Lung cell lysates showing detection of ~20kDa Survivin protein using Rabbit Anti-Survivin Polyclonal Antibody (SPC-1335). Lane 1: Mouse Lung tissue lysate treated with the immunizing peptide. Lane 2: Mouse Lung tissue lysate. Primary Antibody: Rabbit Anti-Survivin Polyclonal Antibody (SPC-1335) at 1:1000. Predicted/Observed Size: ~20kDa. Rabbit Anti-Survivin Antibody used in Western blot (WB) on Lung cell lysates (SPC-1335)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Other related products
    Other_related_products
  • Gene target
    Survivin ATTO 390
  • Host
    Rabbit, Rabbits
  • isotype filter
    • IgG
  • Short name
    Rabbit Anti- Survivin Polyclonal: ATTO 390
  • label filter
    • ATTO 390
  • technique filter
    • anti-Human
    • Polyclonal
    • Rabbit
    • anti-
    • anti
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Alternative technique
    polyclonals, rabbit-anti, antibodies